Generation and Evaluation of Monoclonal Autoantibodies in Systemic Lupus Erythematosis

نویسنده

  • Robabeh Rezaei Poor Department of Microbiology and Immunology, Faculty of Mecicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده مقاله:

Three murine monoclonal anti-ssDNA autoantibodies have been produced by fusion of spleen cells from NZB/NZW F1 hybrid mice with Sp2/0-Ag 14 murine myeloma cells. Specificity studies characterizing monoclonal anti - ssDNA autoantibodies revealed binding patterns for nucleotides. Clone E-4-1 showed preferential binding with mono-di-and polyguanosine. Clones E-4-4 and E-11-1 specitically bound mono-di-and polythymidine. Results of inhibition studies indicated an order of reactivity for each monoclonal autoantiby; polynucleotide) dinucleotide) mononucleotide. 

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عنوان ژورنال

دوره 11  شماره 3

صفحات  122- 131

تاریخ انتشار 1987-09

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